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KMID : 0371320010610030237
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Journal of the Korean Surgical Society
2001 Volume.61 No. 3 p.237 ~ p.246
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Epidermal Growth Factor Dependent Expression of Plasminogen Activator and Plasminogen Activator Inhibitor in Rat Hepatocytes in Primary Culture
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Yoon Wan-Hee
Choi Jung-Hoon
Jung Yeon-Joo
Lee Byung-Hak
Kim Tae-Dong
Song In-Sang
Bae Jin-Sun
Im Kyu
Hwang Byung-Doo
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Abstract
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Purpose
In order to clarify the the role of epidermal growth factor (EGF) in the regulation of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) during liver regeneration, we investigated the EGF-dependent gene expression of PA and
PAI-1
in
rat hepatocytes in primary culture.
Methods
Hepatocytes were isolated from rats using a two step perfusion technique and cultivated in dishes precoated with rat tail collagen. DNA synthesis of the hepatocytes by EGF treatment was measured with 3H-thymidine incorporation. Gene
expression for PAI-1, uPA and tPA was examined using Northern blot hybridization analysis.
Results
EGF treatment increased the 3H-thymidine incorporation of the hepatocytes up to 36 hours and normal polygonal hepatocyte morphology was achi
eved simultaneously. tPA and PAI-1 mRNA were detected in the control hepatocytes. With the EGF treatment, the tPA mRNA level increased with time up to 48 hours, however the PAI-1 mRNA level rapidly increased to 1 hour and then decreased quickly
to
the
control level. On the contrary, uPA mRNA was not detected in hepatocytes with or without treatment of EGF. The EGF-dependent induction of tPA and PAI-1 mRNA was a protein synthesis independent process.
Conclusion
These results suggest that differential expression of tPA and PAI-1 mRNA by EGF in hepatocytes may play an important role in the regulation of liver regeneration. Among PAs, tPA seemed to be more important in EGF dependent growth or regeneration
of
primary hepatocytes in the rat since uPA mRNA was not induced in primary hepatocyte cultures in spite of EGF treatment.
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KEYWORD
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